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Amino acids can be identified and separated using paper chromatography, a cheaper alternative to using TLC. The amino acids need to be stained with ninhydrin to reveal the spots after the run.
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A guide describing some simple, inexpensive and successful methods for carrying out paper and thin layer chromatography, illustrated with examples using biro ink and lipstick.
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Risk assessment information for ninhydrin and ninhydrin solutions or sprays.
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Thin layer chromatography is a quick and effective method of separating pigments within leaves. The pigments run clearly on the chromatogram with little outward spread, allowing Rf values to be calcul.. more
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Also known as acetone. Precautions needed when using it safely in practical work.
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Guidance on general topics including chromatography and electrophoresis, cryogenics, soldering and electrical connections, electrolysis, glass working, glues and adhesives, photography, using pupils a.. more
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It is becoming increasingly difficult to determine the composition of food dyes in an unknown mixture, because formulations have been altered over time, and, as a consequence, the results never match .. more
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This guidance is for devices used in the school laboratory that emit ultraviolet light. Ultraviolet light is classed as optical radiation ranging in wavelength from 100 to 400 nm, below (in wavele.. more
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 Please note: This is a temporary resource providing a summary Contents List of all recipe sheets published to date. RB 000 is a working document, subject to frequent changes as our guidance is update.. more
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An invaluable practical guide on how to safely undertake a range of visually and intellectually appealing chemical reactions. Includes alternatives to some traditional activities such as the thermit r.. more
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