CLEAPSS Supporting practical science and technology
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Amino acids can be identified and separated using paper chromatography, a cheaper alternative to using TLC. The amino acids need to be stained with ninhydrin to reveal the spots after the run.
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Precautions needed when using iodine or iodine solutions (iodine dissolved in potassium iodide or other solvents) i practical activities.
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Thin layer chromatography is a quick and effective method of separating pigments within leaves. The pigments run clearly on the chromatogram with little outward spread, allowing Rf values to be calcul.. more
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Using common dyes and indicators safely in practical activities. Many such compounds , or their solvents, are hazardous but are used in such small amounts and in a very controlled way so as to limit e.. more
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It is becoming increasingly difficult to determine the composition of food dyes in an unknown mixture, because formulations have been altered over time, and, as a consequence, the results never match .. more
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No longer used much in practical work because of hazards. Includes dichloromethane (methylene dichloride), trichloromethane (chloroform), tetrachloromethane (carbon tetrachloride), 1,1,1-trichloroetha.. more
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This demonstration shows preparation of nylon, where solutions of 1,6- diaminohexane in water and an acid chloride in an immiscible solvent are brought together. The nylon polymer forms at the interfa.. more
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The CLEAPSS Helpline receives a number of calls about the teaching of science practical work in non-lab environments. In the normal running of a school we advise against doing this due to additional r.. more
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A guide describing some simple, inexpensive and successful methods for carrying out paper and thin layer chromatography, illustrated with examples using biro ink and lipstick.
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Guidance on general topics including chromatography and electrophoresis, cryogenics, soldering and electrical connections, electrolysis, glass working, glues and adhesives, photography, using pupils a.. more
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