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Guidance for the preparation and use of DNSA (or DNS) reagent for reducing sugars. The reagent may be used qualitatively or quantitatively (colorimetric method).
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Guidance for the preparation of Brady's test reagent from 2,4-dinitrophenylhydrazine.
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Guidance on preparing and using Benedict's reagent (used to test for reducing sugars). 
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The ‘shelf-life’ of a reagent depends on its chemical properties, how it is stored and how frequently the container is opened, thus exposing the reagent to air, moisture and other contaminants. Over t.. more
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Guidance on the preparation and use of Benedict's reagent for the quantitative analysis of reducing sugars. Outline methods for use of the reagent in volumetric and colorimetric procedures are briefly.. more
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Although both aldehydes and ketones contain the carbonyl functional group (-C=O), only aldehydes (or reducing sugars) give a positive ‘silver mirror’ test result with Tollen’s reagent. This small-scal.. more
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Brief risk assessment guidance and experimental notes for activities involving the use of 2,4-dinitrophenylhydrazine (Hazcard 30).
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Index to currently published guidance on the preparation of specific solutions and other reagents. Includes a summary Contents List giving the dates of the most recent versions of recipes. Note: this .. more
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Risk assessment information for 2,4-dintrophenylhydrazine (2,4-DNPH) which is used to make Brady's reagent (for identifying carbonyl compounds). Also, information about phenylhydrazine.
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Updated 01 Nov 2019. Please note: This is a temporary resource providing a summary Contents List of all recipe sheets published to date. RB 000 is a working document, subject to frequent changes as ou.. more
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